| 128 | Lily Perrin (GCW) def. Da Silva, Luana (CARTHAGE) 6-0, 6-0 |
| 128 | Fuchs, Cecile (EC) def. Moen, Mallory (CUW) 6-0, 6-2 |
| 128 | Emily Norman (GACW) def. Morgan Graf (UWL) 6-2, 4-6, 1-0 (12-10) |
| 128 | Raquel Egge (CC-W) def. Sarah Schirm (CENW) 6-2, 6-0 |
| 128 | Rachel Lindrud (GACW) def. Sydney Bell (CAR) 7-6 (7-3), 6-2 |
| 128 | Sofia Sackett (LUTW) def. Lindsey Henderson (UWEC-W) 6-4, 6-3 |
| 128 | Maya Callaway (UWS) def. Elena Bourget (UWEC-W) 6-3, 6-0 |
| 128 | Julia Maier (CSS) def. Courtney Day (UWW-W) 6-2, 6-4 |
| 128 | Sydney Rott (BUW) def. Zoie Becker (SMU) 6-2, 6-0 |
| 128 | Renata Hernandez (GACW) def. Katie Larson (CENW) 6-2, 6-1 |
| 128 | Meadow LaDuke (WARWT) def. Elsa Joly (SCU2122) 6-7 (4-7), 6-3, 1-0 (10-3) |
| 128 | Ramsey, Sam (CARTHAGE) def. Kennedy Morgan (SMU) 6-2, 6-2 |
| 128 | LaBelle, Ashley (CARROLL) def. Alexa Brooks (UWEC-W) 7-5, 6-2 |
| 128 | Phillips, Cheyanne (CUW) def. Molly Rueth (UWW-W) 6-2, 7-6 (7-2) |
| 128 | Samantha Fuchs (UWEC-W) def. Elise Bierbaum (BUW) 6-3, 6-2 |
| 128 | Kaitlyn Kalinka (UWL) def. Meg Ulrich (MAC) 6-3, 7-5 |
| 128 | Halle Rosentreter (GACW) def. Amanye Reynolds (MAC) 6-2, 3-6, 1-0 (10-5) |
| 128 | Madalyn Bigelow (UWW-W) def. Verbauwhede, Ally (EC) 6-4, 6-4 |
| 128 | Alli Laux (GACW) def. Budewitz, Ricki (CUW) 7-5, 7-5 |
| 128 | Jenna Forknell (CC-W) def. Zenia Barretto (UWW-W) 5-7, 6-3, 1-0 (10-8) |
| 128 | Elaine Franta (UWEC-W) def. Barbara Covek (UWSP) 6-2, 6-2 |
| 128 | Utsah Kalra (GCW) def. Ana Olimpio (LUTW) 7-6 (8-6), 6-2 |
| 128 | Hayley Trebil (GACW) def. Emma Thole (BUW) 6-7 (5-7), 6-4, 1-0 (10-8) |
| 128 | Sydney Presler (UWEC-W) def. Hannah Riegle (UWL) 6-3, 4-6, 1-0 (10-7) |
| 128 | Megan Karrow (GACW) def. Bronwyn Metcalf (CENW) 4-6, 6-1, 1-0 (10-6) |
| 128 | Samuta, Ashley (CUW) def. Kellie Ha (UWW-W) 6-4, 5-7, 1-0 (10-5) |
| 128 | Sophia de los Reyes (GCW) def. Susi Hu (UWL) 6-2, 6-0 |
| 128 | Natalia Smith (LUTW) def. Madilyn Jankila (CSS) 6-1, 6-2 |
| 128 | Sofia Walker (SCU2122) def. Rylee Schurman (BUW) 6-2, 6-3 |
| 128 | Emily King (SIMW) def. Kaytlyn Syverson (UWS) 6-2, 6-0 |
| 128 | Maggie Frye (SMU) def. Carew, Alyssa (EC) 6-3, 7-6 (7-4) |
| 64 | Grace Riermann (BUW) def. Lily Perrin (GCW) 6-4, 6-0 |
| 64 | Fuchs, Cecile (EC) def. Sydney Douglas (GACW) 6-1, 6-1 |
| 64 | Ioanna Giannakou (GCW) def. Emily Norman (GACW) 7-6 (7-3), 3-6, 1-0 (11-9) |
| 64 | Raquel Egge (CC-W) def. Amalin Sorajja (MAC) 5-7, 6-3, 1-0 (10-7) |
| 64 | Autumn Bruno (UWW-W) def. Rachel Lindrud (GACW) 6-0, 6-2 |
| 64 | Sofia Sackett (LUTW) def. Lily Fredericks (CSB) 6-2, 6-2 |
| 64 | Tasha Bailey (UWL) def. Maya Callaway (UWS) 6-0, 6-0 |
| 64 | Anna Wanek (SIMW) def. Julia Maier (CSS) 6-2, 6-0 |
| 64 | Kelsey Dorr (SCU2122) def. Sydney Rott (BUW) 6-3, 6-2 |
| 64 | Shelby Cook (LUTW) def. Renata Hernandez (GACW) 6-1, 6-3 |
| 64 | Anna Stutz (GACW) def. Meadow LaDuke (WARWT) 6-1, 6-1 |
| 64 | Erin Jarvis (GCW) def. Ramsey, Sam (CARTHAGE) 6-0, 7-5 |
| 64 | Sabrina Tang (GCW) def. LaBelle, Ashley (CARROLL) 6-0, 6-0 |
| 64 | Madison Schultz (COE-W) def. Phillips, Cheyanne (CUW) 6-4, 6-0 |
| 64 | Cassie Lee (UWW-W) def. Samantha Fuchs (UWEC-W) 7-5, 6-3 |
| 64 | Yuki Oda (GACW) def. Kaitlyn Kalinka (UWL) 6-1, 6-0 |
| 64 | Halle Rosentreter (GACW) def. Olivia Kraft (UWW-W) 6-4, 1-6, 1-0 (11-9) |
| 64 | Irene Agusti (GCW) def. Morgan Nelson (UWEC-W) 6-0, 6-2 |
| 64 | Madalyn Bigelow (UWW-W) def. Alisa Makatsaria (CAR) 6-4, 1-6, 1-0 (10-8) |
| 64 | Apostoli, Giana (CARTHAGE) def. Alli Laux (GACW) 6-4, 5-7, 1-0 (11-9) |
| 64 | Natalie Peterson (SMU) def. Jenna Forknell (CC-W) 6-4, 5-7, 1-0 (10-2) |
| 64 | Ashley Tarrolly (CSB) def. Elaine Franta (UWEC-W) 6-0, 6-2 |
| 64 | Utsah Kalra (GCW) def. Reilee Schepper (SCU2122) 6-0, 6-0 |
| 64 | Kimberly Steinert (UWL) def. Hayley Trebil (GACW) 6-2, 6-1 |
| 64 | Mira Manchanda (GCW) def. Sydney Presler (UWEC-W) 6-0, 6-0 |
| 64 | Gianna Nikoleit (UWW-W) def. Megan Karrow (GACW) 6-4, 7-5 |
| 64 | Samuta, Ashley (CUW) def. Sasha Manojlovic (CARTHAGE) 6-0, 6-1 |
| 64 | Simona Potockova (GACW) def. Sophia de los Reyes (GCW) 6-1, 7-6 (7-1) |
| 64 | Natalia Smith (LUTW) def. Gaedtke, Maya (CARROLL) 6-3, 6-4 |
| 64 | Sydney Ellison (MAC) def. Sofia Walker (SCU2122) 6-2, 6-2 |
| 64 | Clare Palen (UWEC-W) def. Emily King (SIMW) 6-2, 6-0 |
| 64 | Mary Frances Hose (CAR) def. Maggie Frye (SMU) 6-0, 6-0 |
| 32 | Grace Riermann (BUW) def. Fuchs, Cecile (EC) 6-2, 6-1 |
| 32 | Ioanna Giannakou (GCW) def. Raquel Egge (CC-W) 6-1, 6-4 |
| 32 | Autumn Bruno (UWW-W) def. Sofia Sackett (LUTW) 6-3, 6-1 |
| 32 | Tasha Bailey (UWL) def. Anna Wanek (SIMW) 7-5, 6-3 |
| 32 | Kelsey Dorr (SCU2122) def. Shelby Cook (LUTW) 6-1, 6-3 |
| 32 | Anna Stutz (GACW) def. Erin Jarvis (GCW) 6-4, 6-2 |
| 32 | Sabrina Tang (GCW) def. Madison Schultz (COE-W) 6-2, 6-0 |
| 32 | Cassie Lee (UWW-W) def. Yuki Oda (GACW) 6-2, 6-3 |
| 32 | Irene Agusti (GCW) def. Halle Rosentreter (GACW) 6-0, 6-2 |
| 32 | Apostoli, Giana (CARTHAGE) def. Madalyn Bigelow (UWW-W) 6-2, 6-4 |
| 32 | Ashley Tarrolly (CSB) def. Natalie Peterson (SMU) 6-3, 6-3 |
| 32 | Kimberly Steinert (UWL) def. Utsah Kalra (GCW) 3-6, 6-3, 1-0 (10-0) |
| 32 | Gianna Nikoleit (UWW-W) def. Mira Manchanda (GCW) 7-6 (9-7), 6-1 |
| 32 | Simona Potockova (GACW) def. Samuta, Ashley (CUW) 6-1, 6-0 |
| 32 | Natalia Smith (LUTW) def. Sydney Ellison (MAC) 6-1, 6-3 |
| 32 | Mary Frances Hose (CAR) def. Clare Palen (UWEC-W) 6-1, 6-0 |
| 16 | Grace Riermann (BUW) def. Ioanna Giannakou (GCW) 6-1, 6-3 |
| 16 | Autumn Bruno (UWW-W) def. Tasha Bailey (UWL) 6-2, 6-1 |
| 16 | Kelsey Dorr (SCU2122) def. Anna Stutz (GACW) 6-1, 6-0 |
| 16 | Sabrina Tang (GCW) def. Cassie Lee (UWW-W) 6-4, 1-6, 1-0 (10-8) |
| 16 | Irene Agusti (GCW) def. Apostoli, Giana (CARTHAGE) 6-4, 6-1 |
| 16 | Kimberly Steinert (UWL) def. Ashley Tarrolly (CSB) 6-0, 6-2 |
| 16 | Simona Potockova (GACW) def. Gianna Nikoleit (UWW-W) 6-4, 1-6, 1-0 (10-6) |
| 16 | Mary Frances Hose (CAR) def. Natalia Smith (LUTW) 6-1, 6-2 |
| 8 | Autumn Bruno (UWW-W) def. Grace Riermann (BUW) 6-4, 6-3 |
| 8 | Sabrina Tang (GCW) def. Kelsey Dorr (SCU2122) 6-1, 6-1 |
| 8 | Irene Agusti (GCW) def. Kimberly Steinert (UWL) 6-4, 4-6, 6-2 |
| 8 | Mary Frances Hose (CAR) def. Simona Potockova (GACW) 6-0, 6-3 |
| 4 | Sabrina Tang (GCW) def. Autumn Bruno (UWW-W) 6-1, 6-1 |
| 4 | Mary Frances Hose (CAR) def. Irene Agusti (GCW) 6-2, 5-7, 6-3 |
| 2 | Sabrina Tang (GCW) def. Mary Frances Hose (CAR) by default |
| - | Consolation A |
| C32A | Peyton Buerman (SMU) def. Jennifer Larson (CENW) 8-5 |
| C32A | Rylee Schurman (BUW) def. Rose Han (GACW) 8-0 |
| C32A | Sydney Presler (UWEC-W) def. Ava Helmer (HU) 8-0 |
| C16A | Sydney Douglas (GACW) def. Peyton Buerman (SMU) 8-0 |
| C16A | Susi Hu (UWL) def. Grace Buswell (UNW) 8-0 |
| C16A | Kunze, Maya (CUW) def. Sydney Hubbard (HU) 8-3 |
| C16A | Rylee Schurman (BUW) def. Leah Miller (UWEC-W) 8-6 |
| C16A | Sydney Presler (UWEC-W) def. Taylor Hopkins (HU) 8-0 |
| C16A | Katie Larson (CENW) def. Rachel Fanciullo (GACW) 8-5 |
| C16A | Papp, Rachel (CARROLL) def. Sofia Holmes (SMU) 8-3 |
| C16A | Phillips, Cheyanne (CUW) def. Emma Thole (BUW) 8-1 |
| C8A | Sydney Douglas (GACW) def. Susi Hu (UWL) 8-2 |
| C8A | Rylee Schurman (BUW) def. Kunze, Maya (CUW) 8-7 (8-6) |
| C8A | Sydney Presler (UWEC-W) def. Katie Larson (CENW) 8-6 |
| C8A | Phillips, Cheyanne (CUW) def. Papp, Rachel (CARROLL) 8-0 |
| C4A | Rylee Schurman (BUW) def. Sydney Douglas (GACW) 8-6 |
| C4A | Phillips, Cheyanne (CUW) def. Sydney Presler (UWEC-W) 8-6 |
| C2A | Phillips, Cheyanne (CUW) def. Rylee Schurman (BUW) 8-5 |
| - | Consolation B |
| C32B | Jersi McAlpine (HU) def. Kara Hand (SMU) 8-0 |
| C32B | Manders, Karina (EC) def. Ashley Barker (GACW) 8-1 |
| C32B | Bronwyn Metcalf (CENW) def. Cady Peterson (BUW) 8-2 |
| C16B | Samantha Fuchs (UWEC-W) def. Jersi McAlpine (HU) 8-1 |
| C16B | Sarah Schirm (CENW) def. Manders, Karina (EC) 8-1 |
| C16B | Sara Borchard (GACW) def. Rayna Baumgarn (UNW) 8-0 |
| C16B | Elise Bierbaum (BUW) def. Ella Boomgaarden (SMU) 8-0 |
| C16B | Verbauwhede, Ally (EC) def. Elise Jarchow (UNW) 8-5 |
| C16B | Ashley Renwick (SMU) def. Frerking, Karen (CUW) 8-7 (7-3) |
| C16B | Michaela Pinto (COE-W) def. Luna van Tol (LUTW) 8-6 |
| C16B | Bronwyn Metcalf (CENW) def. Emily Norman (GACW) 8-7 (7-2) |
| C8B | Samantha Fuchs (UWEC-W) def. Sarah Schirm (CENW) 8-0 |
| C8B | Elise Bierbaum (BUW) def. Sara Borchard (GACW) 8-3 |
| C8B | Verbauwhede, Ally (EC) def. Ashley Renwick (SMU) 8-5 |
| C8B | Bronwyn Metcalf (CENW) def. Michaela Pinto (COE-W) 8-2 |
| C4B | Samantha Fuchs (UWEC-W) def. Elise Bierbaum (BUW) 8-2 |
| C4B | Bronwyn Metcalf (CENW) def. Verbauwhede, Ally (EC) 8-2 |
| C2B | Samantha Fuchs (UWEC-W) def. Bronwyn Metcalf (CENW) 8-3 |
| - | Consolation A vs. B |
| CF | Samantha Fuchs (UWEC-W) def. Phillips, Cheyanne (CUW) 8-2 |
